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streptavidin conjugated to fitc  (Vector Laboratories)


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    Structured Review

    Vector Laboratories streptavidin conjugated to fitc
    Streptavidin Conjugated To Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+streptavidin/Fluorescein+Streptavidin/pmc12755826-140-5-10
    Average 94 stars, based on 512 article reviews
    streptavidin conjugated to fitc - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-human sarcomeric actin (Dako, Santa Clara, CA, USA, cat. # M0874), mouse anti-human desmin (cat. # NCL-L-DES-DERII), mouse anti-human troponin T (both from Leica, Wetzlar, Germany, cat. # NCL-TROPT), rabbit anti-human anti-connexin 43 (Santa cruz biotechnology, Dallas, TX, USA, cat. # 101660), biotinylated lycopersicon esculentum Lectin (Vector Laboratories, Burlingame, CA, USA, cat. # B-1175) and mouse anti-human smooth muscle actin (Biogenex, Fremont, CA, USA, cat. # AM128-5M) antibodies were used, and a secondary goat anti-mouse antibody conjugated with FITC (Kallestad, Chaska, MN, USA, cat. # 400288) and goat antirabbit conjugated with FITC (Vector Laboratories, Burlingame, CA, USA, cat. # FI-1000) were added. .. Anti-Biotinylated Lectin was labeled with FITC-conjugated streptavidin (Vector Laboratories, Burlingame, CA, USA, cat. # SA-5001). .. Direct immunofluorescence was performed, incubating AT-Muse cells at P4 with primary rat anti-human SSEA3 conjugated with FITC (Invitrogen, Waltham, MA, USA, cat. # MA1020-D488).

    Article Title: Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-human sarcomeric actin (Dako, Santa Clara, CA, USA, cat. # M0874), mouse anti-human desmin (cat. # NCL-L-DES-DERII), mouse anti-human troponin T (both from Leica, Wetzlar, Germany, cat. # NCL-TROPT), rabbit anti-human anti-connexin 43 (Santa cruz biotechnology, Dallas, TX, USA, cat. # 101660), biotinylated lycopersicon esculentum Lectin (Vector Laboratories, Burlingame, CA, USA, cat. # B-1175) and mouse anti-human smooth muscle actin (Biogenex, Fremont, CA, USA, cat. # AM128-5M) antibodies were used, and a secondary goat anti-mouse antibody conjugated with FITC (Kallestad, Chaska, MN, USA, cat. # 400288) and goat anti-rabbit conjugated with FITC (Vector Laboratories, Burlingame, CA, USA, cat. # FI-1000) were added. .. Anti-Biotinylated Lectin was labeled with FITC-conjugated streptavidin (Vector Laboratories, Burlingame, CA, USA, cat. # SA-5001). .. Direct immunofluorescence was performed, incubating AT-Muse cells at P4 with primary rat anti-human SSEA3 conjugated with FITC (Invitrogen, Waltham, MA, USA, cat. # MA1-020-D488).

    Staining:

    Article Title: Inhibitory immunoreceptors CD300a and CD300lf cooperate to regulate mast cell activation.
    Article Snippet: .. These transfectants were then stained with rabbit anti-phospholyrated Syk (Tyr525/526) polyclonal antibody (dilution at 1:1000) (#2711s, Cell signaling, Danvers, Massachusetts, USA) overnight at 4�C, washed with PBS three times, and stained with anti-rabbit IgG Alexa 647, FITC-conjugated streptavidin, and 4',6-diamidino-2phenylindole (DAPI) (H-1200, Vector Laboratories, ow nloaded from https://academ ic.oup.com /jim m unol/advance-article/doi/10.1093/jim m un/vkae030/8050800 by guest on 14 M arch 2025 Burlingame, California, USA) for 2h at RT, and analyzed by laser scanning confocal microscopy using the FV-ASW software. .. For human MCs degranulation, cells were seeded at 3 × 106/mL and sensitized with 0.5 ug/ml of human IgE (SigmaAldrich) for 1 h, washed with Tyrode’s buffer, and plated at 1 × 105 cells/well in a 96-well plate.

    Confocal Microscopy:

    Article Title: Inhibitory immunoreceptors CD300a and CD300lf cooperate to regulate mast cell activation.
    Article Snippet: .. These transfectants were then stained with rabbit anti-phospholyrated Syk (Tyr525/526) polyclonal antibody (dilution at 1:1000) (#2711s, Cell signaling, Danvers, Massachusetts, USA) overnight at 4�C, washed with PBS three times, and stained with anti-rabbit IgG Alexa 647, FITC-conjugated streptavidin, and 4',6-diamidino-2phenylindole (DAPI) (H-1200, Vector Laboratories, ow nloaded from https://academ ic.oup.com /jim m unol/advance-article/doi/10.1093/jim m un/vkae030/8050800 by guest on 14 M arch 2025 Burlingame, California, USA) for 2h at RT, and analyzed by laser scanning confocal microscopy using the FV-ASW software. .. For human MCs degranulation, cells were seeded at 3 × 106/mL and sensitized with 0.5 ug/ml of human IgE (SigmaAldrich) for 1 h, washed with Tyrode’s buffer, and plated at 1 × 105 cells/well in a 96-well plate.

    Software:

    Article Title: Inhibitory immunoreceptors CD300a and CD300lf cooperate to regulate mast cell activation.
    Article Snippet: .. These transfectants were then stained with rabbit anti-phospholyrated Syk (Tyr525/526) polyclonal antibody (dilution at 1:1000) (#2711s, Cell signaling, Danvers, Massachusetts, USA) overnight at 4�C, washed with PBS three times, and stained with anti-rabbit IgG Alexa 647, FITC-conjugated streptavidin, and 4',6-diamidino-2phenylindole (DAPI) (H-1200, Vector Laboratories, ow nloaded from https://academ ic.oup.com /jim m unol/advance-article/doi/10.1093/jim m un/vkae030/8050800 by guest on 14 M arch 2025 Burlingame, California, USA) for 2h at RT, and analyzed by laser scanning confocal microscopy using the FV-ASW software. .. For human MCs degranulation, cells were seeded at 3 × 106/mL and sensitized with 0.5 ug/ml of human IgE (SigmaAldrich) for 1 h, washed with Tyrode’s buffer, and plated at 1 × 105 cells/well in a 96-well plate.

    Immunofluorescence:

    Article Title: Different isolation approaches lead to diverse glycosylated extracellular vesicle populations
    Article Snippet: .. Single immunofluorescence with the biotinylated L-PHA lectin (Vector Laboratories, Burlingame, CA, USA) was performed by blocking with PBS, 10% BSA, the lectin diluted 1/500 in PBS overnight at 4oC and FITC-conjugated streptavidin diluted 1/1000 in PBS. .. DAPI was used for nuclear staining at RT for 10 min and slides were mounted in VectaShield (Vector Laboratories, Burlingame, CA, USA).

    Blocking Assay:

    Article Title: Different isolation approaches lead to diverse glycosylated extracellular vesicle populations
    Article Snippet: .. Single immunofluorescence with the biotinylated L-PHA lectin (Vector Laboratories, Burlingame, CA, USA) was performed by blocking with PBS, 10% BSA, the lectin diluted 1/500 in PBS overnight at 4oC and FITC-conjugated streptavidin diluted 1/1000 in PBS. .. DAPI was used for nuclear staining at RT for 10 min and slides were mounted in VectaShield (Vector Laboratories, Burlingame, CA, USA).



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